GBC Human Albumin/IgG/Transferrin 3 in 1 Depletion Kit is designed to specifically remove albumin, IgG and transferrin from human serum/plasma for various downstream applications, e.g. 1DE, 2DE, and LC/MS. The affinity medium in the spin column is the resin coupled with duck anti-human albumin, anti-IgG, and anti-transferrin affinity-purified IgYΔFc polyclonal antibodies. Each GBC Human 3 in 1 Depletion Kit contains 2 spin columns packed with 0.32ml affinity resin, 2 empty spin columns, 80ml Equilibration Buffer and 60ml Elution Buffer for optimal resin performance. The depletion spin column can remove greater than 98% of the albumin, IgG and transferrin from an 15 µl serum sample as determined by ELISA and yield about 280-330 µg/sample of total protein after single depletion process.
Features and Benefits
ü Efficient removal of albumin, IgG and transferrin with minimal loss of other serum biomarkers
ü Fast and simple procedure, applicable for multiple processing
ü Not bind mammalian complement factors, rheumatoid factor, IgM, Fc receptor
ü Applications: 1D or 2D electrophoresis, LC/MS, etc.
Figure 1. Human serum proteins after depletion of albumin, IgG and transferrin using GBC Human Albumin/IgG/Transferrin 3 in 1 Depletion Spin Column. The serum proteins were separated by 5-15% (A) non-reducing and (B) reducing SDS-PAGE and stained by Coomassie Brilliant Blue R. M, marker; Lane 1, serum proteins; Lane 2, depleted serum from 15µl serum; Lane 3, eluted proteins from 15µl serum. [(1)IgG (2)Transferrin (3)Albumin (4)IgG heavy-chain (5)IgG light-chain]
Click HD-0301-1.5KT the package insert of GBC Human Albumin/IgG/Transferrin 3 in 1 Depletion Kit.
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