Revolutionizing Biomarker Discovery with the Good Biotech 3 in 1 Depletion Column

Rapid, selective removal of albumin, IgG, and transferrin to reveal the hidden plasma proteome

Harnessing pioneering affinity-purified duck IgY(ΔFc) polyclonal antibodies, the Good Biotech 3 in 1 Depletion Column line delivers a single-step prefractionation that uncovers low-abundance biomarkers, sharpens 2-DE resolution, and boosts LC/MS sensitivity—all while preserving other clinically relevant proteins.

Why Depletion Columns Matter

High‑abundance proteins can mask critical biomarkers in plasma, leading to:

  • Dynamic-range suppression: High-abundance proteins mask critical low-abundance species.
  • Heterogeneity artifacts: Charge microvariants smear spots on 2-DE gels.
  • Spot crowding: Protein clusters at 45–80 kDa and pI 4.5–6 reduce resolution.

Solution: Efficiently remove albumin, IgG, and transferrin to expose the full plasma proteome.

Advantages of Affinity-Purified IgY(ΔFc) Antibody-Based Depletion Columns

Our three formats—Spin Column, Spin-Kit, and Gravity-Flow Column—employ resin beads, each coupled to duck IgY(ΔFc) antibodies specific for human albumin, IgG, and transferrin:

  • Resin volumes: 0.32 mL per spin-format column; 4 mL per gravity-flow column
  • Workflow flexibility: Sample sizes from 15 µL to 200 µL
  • > 98 % depletion efficiency: Single-pass removal of all three targets (SDS-PAGE verified)
  • Exceptional specificity: No binding to complement factors, rheumatoid factor, IgM, or Fc receptors
  • Enhanced epitope coverage: Broader recognition of conserved mammalian protein regions compared to mammalian IgG

Key Features

  • Selective capture: Exclusively targets albumin, IgG, and transferrin
  • Simple operation: Spin-down or gravity-driven protocol—no pumps required
  • High durability:
    • Spin formats: > 20 cycles
    • Gravity-flow format: > 200 cycles
  • Three formats to suit any workflow:
    • Spin Column (REF HD-0301-1.5SC)
    • Spin-Kit (REF HD-0301-1.5KT)
    • Gravity-Flow Column (REF HD-0301-10GFC)
  • Broad compatibility: 1D/2D electrophoresis, LC/MS, Western blotting, immunoassays

  •           Customizable Affinity-Purified IgY(ΔFc) Ab-based Depletion Columns

Product name
 
(REF)
Good Biotech 3 in 1 Depletion Column (Spin‑Format)
Good Biotech 3 in 1 Depletion Column 
(Spin‑Kit)
Good Biotech 3 in 1 Depletion Column (Gravity‑Flow)
Depletion Method                        
Spin-down
Spin-down
Gravity-flow
Column Size
1.5 mL
1.5 mL
10 mL
Resin Beds
0.32 mL
0.32 mL
4 mL
Sample Volume
15 µl
15 µl
200 µl
Depletion Rate:
      Albumin
          IgG
    Transferrin
>98%
>98%
>98%
Enriched Protein Yield     (per column/run)
280-330 µg
280-330 µg
3.0-3.4 mg
Reuse Cycles*
> 20
> 20
> 200
Package
Spin Column x 2
Spin Column x 2
Equilibration Buffer 80mL
Elution Buffer 60mL
Depletion Column x 1
*Cycle counts may vary with operating conditions.
 

Custom Solutions

Design your ideal depletion column. Select the format, resin volume, and flow method that match your sample size and throughput.

✉️ Contact us: orders@good-biotech.com