REF HD-0301-1.5KT
All-in-One Spin-Kit: 3 in 1 Depletion Workflow
Complete spin-column system with equilibration & elution buffers for batch processing of 15 µL samples
Overview
A turnkey kit for parallel depletion of albumin, IgG, and transferrin from multiple 15 µL serum/plasma samples. Includes all reagents for efficient, reproducible prefractionation.
Kit Contents
- 2 × pre-packed spin columns (0.32 mL duck IgY(ΔFc) resin)
- 2 × empty spin columns
- 80 mL Equilibration Buffer
- 60 mL Elution Buffer
Performance Highlights
- > 98 % depletion efficiency of albumin, IgG, and transferrin (SDS PAGE-verified)
- Yield: 280–330 µg enriched protein per run
- Reusability: > 20 cycles per resin bed
Key Benefits
- Broad epitope recognition: Captures conserved regions better than mammalian IgG
- No off-target loss: Preserves complement factors, rheumatoid factor, IgM, and Fc receptors
- Scalable workflow: Process samples individually or in parallel
Figure 1. Human serum proteins after depletion of albumin, IgG, and transferrin using Good Biotech Human Albumin/IgG/Transferrin 3 in 1 Depletion Spin Column. The serum proteins were separated by 5-15% (A) non-reducing and (B) reducing SDS-PAGE and stained by Coomassie Brilliant Blue R. M, marker; Lane 1, serum proteins; Lane 2, depleted serum from 15µl serum; Lane 3, eluted proteins from 15µl serum. [(1)IgG (2)Transferrin (3)Albumin (4)IgG heavy-chain (5)IgG light-chain]
Visit the related page:
⇒Good Biotech 3 in 1 Depletion Column (Spin Format)
⇒Good Biotech 3 in 1 Depletion Column (Gravity-Flow)
