REF HD-0301-10GFC
High-Capacity Throughput: 3 in 1 Gravity-Flow Depletion Column
Pump-free, gravity-driven depletion of albumin, IgG, and transferrin from 200 µL plasma for mg-scale recovery
Overview
A large-volume, gravity-flow column for depletion of albumin, IgG, and transferrin from 200 µL human serum or plasma, enabling milligram-scale enriched protein for in-depth analyses.
Affinity Medium
4 mL resin per column, each bead coupled to duck IgY(ΔFc) polyclonal antibodies selective for human albumin, IgG and transferrin—combining high capacity with ultra-low nonspecific binding.
Performance Highlights
- > 98 % depletion efficiency of albumin, IgG, and transferrin (SDS PAGE-verified)
- Yield: 3.0–3.4 mg enriched protein per run
- Reusability: > 200 gravity-flow cycles
Key Benefits
- High throughput: Process larger volumes without pumps or specialized equipment
- Exceptional specificity: No interaction with complement, rheumatoid factor, IgM or Fc receptors
- Versatile compatibility: 1D/2D electrophoresis, LC/MS, Western blotting, immunoassays
Figure 1. Human serum proteins, after depletion of albumin, IgG, and transferrin processed GBC 3 in 1 Depletion Column. 10µg serum proteins of flow-through and elution fractions were separated by 5-15% (A) non-reducing (B) reducing SDS-PAGE and stained by Coomassie Brilliant Blue R. M, marker. Lane 1, serum proteins. Lane 2-3, depleted serum from 200µl serum. Lane 4, eluted proteins. [(1)IgG, (2)transferrin, (3)albumin, (4)IgG-heavy chain, (5)IgG-light chain]
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